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The claim of “Antibacterial” or “Kill 99.9% germs” is commonly found on hand cleansing gel, hand wash and household cleaners. This efficacy claim shall be supported by scientific testing results so that consumers can make their right choice. With our expertise in chemical and microbial testing and worldwide network, SGS can help you to substantiate your claim in the market.

a lady using a hand sanitizer for cleaning hands

Test method according to EN 1265 and EN 1650

EN 1276 and EN 1650 are well known standard methods to evaluate, respectively, bactericidal and fungicidal/yeasticidial activities. Both methods are applicable to hand hygiene products and disinfectants (e.g. household cleaner) [note 1]. Briefly, your product effectiveness will be evaluated under a simulated dirty condition by the addition of bovine albumin protein.

Note 1: EN 1276 and EN 1650 is not applicable to medical use product and products to be used on living tissues, except hand hygiene products.

A summary of test methods between EN 1276 and EN 1650


Bactericidal Activity Fungicidal / Yeasticidal Activity
Standard Method EN 1276 EN 1650
Test organisms
  • Pseudomonas aeruginosa
  • Staphylococcus aureus
  • Enterocococcus hirae
  • Escherichia coli
  • Candida albicans
  • Aspergillus niger
Obligatory test conditions 20 ± 1°C
1 min ± 5 s for hand hygiene products
5 min ± 10s for other products
20 ± 1°C
15 min ± 10s
Functions Evaluate the bactericidal activity under simulated actual use conditions Evaluate the fungicidal / yeasticidal activity under simulated actual use conditions

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